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Download 9reat stand by me em karaokeIn vitro susceptibility and mutational frequency.
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Monte Carlo simulations from previously developed population PK models in adults and children were conducted to explore the probability of target attainment (PTA) for teicoplanin dosage regimens against the current EUCAST WT susceptibility range.
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The rate of growth is a balance between bacterial growth and death.
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Download 9reat stand by me em karaokeFor the paediatric simulations, we used the population PK estimates and covariance matrix from a previously developed PK model in children.
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Download 9reat stand by me em karaokeFor the adult simulations we used the population PK estimates and covariance matrix from a previously developed population PK model in adults.
The coefficient of determination and visual examination of the fit were used to discriminate the various PD indices.
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Download 9reat stand by me em karaokeThe mean and SD for a group of three mice was then calculated.
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These simulated patients were used to examine the outcomes of candidate regimens of teicoplanin.
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The mean value of the bacterial burden from the left and right thighs from each mouse was determined.
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Pharmacodynamics of teicoplanin against MRSA.
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In vitro PK and PD studies.
Quantitative Microsphere System (QMS) technology and was implemented on an automated analyser (Abbott Architect ci4100).
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In vitro PD studies.
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We considered this relevant to evaluate the impact of frequency on AUC and Cmin attained drug exposures.
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Pharmacodynamics and Therapeutics, Institute of Translational Medicine, University of Liverpool, Liverpool, UK2Alder Hey Children's NHS Foundation Trust, Liverpool, UK.
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Download 9reat stand by me em karaokeWe finally bridged the experimental results to patients to reflect on the adequacy of current EUCAST in vitro susceptibility breakpoints.
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Monte Carlo simulations and probability of target attainment (PTA) analysis.
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These updated targets are based on small retrospective studies in adults.
Download 9reat stand by me em karaokeWe considered both the antibacterial effect of teicoplanin and the emergence of resistance to teicoplanin as primary study endpoints.
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Total and resistant subpopulations were quantified.
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Cmin at steady state (between days 3 and 4 of treatment) was performed for each dosage regimen for both adults and children.
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PK profile was simulated in the HFIM.
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Download 9reat stand by me em karaokeMonte Carlo simulations were performed with Pmetrics.
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MIC target for efficacy and suppression of resistance.
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PD of teicoplanin to that provided by the HFIM.
Fractional target attainment was calculated for the various regimens.
Each experiment comprised a control arm and three cohorts of mice receiving teicoplanin.
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Pharmacodynamics and Therapeutics, Institute of Translational Medicine, University of Liverpool, Liverpool, UK.
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The MIC for the strain was determined using the EUCAST broth microdilution methodology on three separate occasions.
Applied Pharmacokinetics and Bioinformatics, The Saban Research Institute and The Division of Paediatric Infectious Diseases, Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.
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Download 9reat stand by me em karaokeTeicoplanin is a glycopeptide with a similar spectrum of antibacterial activity to vancomycin.
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Three mice were used per dose timepoint.
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